University of Massachusetts Medical School Faculty Publications
Title
An Estradiol-Inducible Promoter Enables Fast, Graduated Control of Gene Expression in Fission Yeast
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
2-17-2017
Document Type
Article Preprint
Disciplines
Cell Biology | Fungi | Genetic Phenomena | Hormones, Hormone Substitutes, and Hormone Antagonists
Abstract
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S. pombe. We show that this promoter system, termed Z3EV, turns on quickly, can reach a maximal induction of 20 fold, and exhibits a linear dose response over its entire induction range, with few off target effects. We demonstrate the utility of this system by regulating the mitotic inhibitor Wee1 to create a strain in which cell size is regulated by β-estradiol concentration. This promoter system will be of great utility for experimentally regulating gene expression in fission yeast.
Keywords
cell biology, Schizosaccharomyces pombe, gene expression, estradiol
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/092338
Source
bioRxiv 092338; doi: https://doi.org/10.1101/092338. Link to preprint on bioRxiv service.
Related Resources
Now published in Yeast doi: 10.1002/yea.3235.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Ohira, Makoto J.; Hendrickson, David G.; McIsaac, R. Scott; and Rhind, Nicholas R., "An Estradiol-Inducible Promoter Enables Fast, Graduated Control of Gene Expression in Fission Yeast" (2017). University of Massachusetts Medical School Faculty Publications. 1554.
https://escholarship.umassmed.edu/faculty_pubs/1554
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Cell Biology Commons, Fungi Commons, Genetic Phenomena Commons, Hormones, Hormone Substitutes, and Hormone Antagonists Commons