Program in Bioinformatics and Integrative Biology
Amino Acids, Peptides, and Proteins | Biochemistry | Genetic Phenomena | Genetics | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
genetics, TRIM-NHL, proteins, NHL-2, CSR-1, HRDE-1, 22G-RNA, germline, gene expression
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license.
DOI of Published Version
bioRxiv 260240; doi: https://doi.org/10.1101/260240. Link to preprint on bioRxiv service.
Boag PR, Davis GM, Tu S, Colson RN, Anderson JW, Gunzburg MJ, Francisco MA, Ray D, Maity T, Wu MZ, Morris QD, Hughes TR, Wilce JA, University of Toronto, Weng Z. (2018). The TRIM-NHL protein NHL-2 is a Novel Co-Factor of the CSR-1 and HRDE-1 22G-RNA Pathways [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/260240. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1543
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This work is licensed under a Creative Commons Attribution 4.0 License.