UMass Chan Medical School Faculty Publications

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Program in Bioinformatics and Integrative Biology

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Article Preprint


Amino Acids, Peptides, and Proteins | Biochemistry | Genetic Phenomena | Genetics | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides


Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.


genetics, TRIM-NHL, proteins, NHL-2, CSR-1, HRDE-1, 22G-RNA, germline, gene expression

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bioRxiv 260240; doi: Link to preprint on bioRxiv service.

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Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.