UMass Chan Medical School Faculty Publications
Title
The TRIM-NHL protein NHL-2 is a Novel Co-Factor of the CSR-1 and HRDE-1 22G-RNA Pathways [preprint]
UMMS Affiliation
Program in Bioinformatics and Integrative Biology
Publication Date
2018-02-05
Document Type
Article Preprint
Disciplines
Amino Acids, Peptides, and Proteins | Biochemistry | Genetic Phenomena | Genetics | Molecular Biology | Nucleic Acids, Nucleotides, and Nucleosides
Abstract
Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
Keywords
genetics, TRIM-NHL, proteins, NHL-2, CSR-1, HRDE-1, 22G-RNA, germline, gene expression
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license.
DOI of Published Version
10.1101/260240
Source
bioRxiv 260240; doi: https://doi.org/10.1101/260240. Link to preprint on bioRxiv service.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Boag PR, Davis GM, Tu S, Colson RN, Anderson JW, Gunzburg MJ, Francisco MA, Ray D, Maity T, Wu MZ, Morris QD, Hughes TR, Wilce JA, University of Toronto, Weng Z. (2018). The TRIM-NHL protein NHL-2 is a Novel Co-Factor of the CSR-1 and HRDE-1 22G-RNA Pathways [preprint]. UMass Chan Medical School Faculty Publications. https://doi.org/10.1101/260240. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1543
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemistry Commons, Genetic Phenomena Commons, Genetics Commons, Molecular Biology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons