University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Publication Date
2017-09-29
Document Type
Article Preprint
Disciplines
Cell Biology | Cellular and Molecular Physiology | Genetic Phenomena
Abstract
In contrast to the well-studied condensation and folding of chromosomes during mitosis, their dynamics in interphase are less understood. We developed a sensitive, multicolor system, CRISPR-Sirius, allowing the real-time tracking of the dynamics of chromosomal loci. We tracked loci kilobases to megabases apart and found significant variation in the inter-locus distances of each pair, indicating differing degrees of DNA contortion. We resolved two distinct modes of dynamics of loci: saltatory local movements as well as translational movements of the domain. The magnitude of both of these modes of movements increased from early to late G1, whereas the translational movements were reduced in early S. The local fluctuations decreased slightly in early S and more markedly in mid-late S. These newly observed movements and their cell cycle-dependence are indicative of a hitherto unrecognized compaction-relaxation dynamic of the chromosomal fiber operating concurrently with changes in the extent of observed genomic domain movements.
Keywords
CRISPR, DNA imaging, CRISPR-Sirius, chromosomal loci, DNA contortion, chromosomes, cell biology
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
10.1101/195966
Source
bioRxiv 195966; doi: https://doi.org/10.1101/195966. Link to preprint on bioRxiv service.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Ma H, Tu L, University of Central Florida, Chung Y, Grünwald D, Zhang S, Pederson T. (2017). CRISPR-Based DNA Imaging in Living Cells Reveals Cell Cycle-Dependent Chromosome Dynamics. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/195966. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1525
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Included in
Cell Biology Commons, Cellular and Molecular Physiology Commons, Genetic Phenomena Commons