University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Neurobiology; Department of Biochemistry and Molecular Pharmacology; Budnik Lab
Publication Date
2017-11-22
Document Type
Article Preprint
Disciplines
Amino Acids, Peptides, and Proteins | Cell Biology | Neuroscience and Neurobiology | Nucleic Acids, Nucleotides, and Nucleosides
Abstract
In eukaryotes, subsets of exported mRNAs are organized into large ribonucleoprotein (megaRNP) granules. How megaRNPs exit the nucleus is unclear, as their diameters are much larger than the nuclear pore complex (NPC) central channel. We previously identified a non-canonical nuclear export mechanism in Drosophila (Speese et al., Cell 2012) and mammals (Ding et al., in preparation), in which megaRNPs exit the nucleus by budding across nuclear envelope (NE) membranes. Here, we present evidence for a similar pathway in the nucleus of the budding yeast S. cerevisiae, which contain morphologically similar granules bearing mRNAs. Wild-type yeast displayed these granules at very low frequency, but this frequency was dramatically increased when the non-essential NPC protein Nup116 was deleted. These granules were not artifacts of defective NPCs; a mutation in the exportin XPO1 (CRM1), in which NPCs are normal, induced similar megaRNP upregulation. We hypothesize that a non-canonical nuclear export pathway, analogous to those observed in Drosophila and in mammalian cells, exists in yeast, and that this pathway is upregulated for use when NPCs or nuclear export are impaired.
Keywords
eukaryotes, nuclear envelope remodeling, mRNA, ribonucleoprotein, S. cerevisiae, Nup116, cell biology
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.
DOI of Published Version
10.1101/224055
Source
bioRxiv 224055; doi: https://doi.org/10.1101/224055. Link to preprint on bioRxiv service.
Journal/Book/Conference Title
bioRxiv
Repository Citation
Ding B, Mirza AM, Ashley JA, Budnik V, Munson M. (2017). Nuclear Export Through Nuclear Envelope Remodeling in Saccharomyces cerevisiae [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/224055. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1519
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Included in
Amino Acids, Peptides, and Proteins Commons, Cell Biology Commons, Neuroscience and Neurobiology Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons