University of Massachusetts Medical School Faculty Publications

UMMS Affiliation

Department of Neurobiology; Department of Biochemistry and Molecular Pharmacology; Budnik Lab

Document Type

Article Preprint

Disciplines

Amino Acids, Peptides, and Proteins | Cell Biology | Neuroscience and Neurobiology | Nucleic Acids, Nucleotides, and Nucleosides

Abstract

In eukaryotes, subsets of exported mRNAs are organized into large ribonucleoprotein (megaRNP) granules. How megaRNPs exit the nucleus is unclear, as their diameters are much larger than the nuclear pore complex (NPC) central channel. We previously identified a non-canonical nuclear export mechanism in Drosophila (Speese et al., Cell 2012) and mammals (Ding et al., in preparation), in which megaRNPs exit the nucleus by budding across nuclear envelope (NE) membranes. Here, we present evidence for a similar pathway in the nucleus of the budding yeast S. cerevisiae, which contain morphologically similar granules bearing mRNAs. Wild-type yeast displayed these granules at very low frequency, but this frequency was dramatically increased when the non-essential NPC protein Nup116 was deleted. These granules were not artifacts of defective NPCs; a mutation in the exportin XPO1 (CRM1), in which NPCs are normal, induced similar megaRNP upregulation. We hypothesize that a non-canonical nuclear export pathway, analogous to those observed in Drosophila and in mammalian cells, exists in yeast, and that this pathway is upregulated for use when NPCs or nuclear export are impaired.

Keywords

eukaryotes, nuclear envelope remodeling, mRNA, ribonucleoprotein, S. cerevisiae, Nup116, cell biology

Rights and Permissions

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.

DOI of Published Version

10.1101/224055

Source

bioRxiv 224055; doi: https://doi.org/10.1101/224055. Link to preprint on bioRxiv service.

Journal/Book/Conference Title

bioRxiv

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

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