RNA Therapeutics Institute
Computational Biology | Genetic Phenomena | Genomics | Nucleic Acids, Nucleotides, and Nucleosides
Recursive splicing, a process by which a single intron is removed from pre-mRNA transcripts in multiple distinct segments, has been observed in a small subset of Drosophila melanogaster introns. However, detection of recursive splicing requires observation of splicing intermediates which are inherently unstable, making it difficult to study. Here we developed new computational approaches to identify recursively spliced introns and applied them, in combination with existing methods, to nascent RNA sequencing data from Drosophila S2 cells. These approaches identified hundreds of novel sites of recursive splicing, expanding the catalog of recursively spliced fly introns by 4-fold. Recursive sites occur in most very long (> 40 kb) fly introns, including many genes involved in morphogenesis and development, and tend to occur near the midpoints of introns. Suggesting a possible function for recursive splicing, we observe that fly introns with recursive sites are spliced more accurately than comparably sized non-recursive introns.
introns, recursive splicing, Drosophila melanogaster, mRNA, genomics
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.
DOI of Published Version
bioRxiv 290007; doi: https://doi.org/10.1101/290007. Link to preprint on bioRxiv service.
Pai AA, Paggi J, Adelman K, Burge CB. (2018). Numerous recursive sites contribute to accuracy of splicing of long introns in flies [preprint]. UMass Chan Medical School Faculty Publications. https://doi.org/10.1101/290007. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1518
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This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License