University of Massachusetts Medical School Faculty Publications
UMMS Affiliation
Department of Neurobiology; Alkema Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Publication Date
2014-04-02
Document Type
Article Preprint
Disciplines
Neuroscience and Neurobiology | Systems Neuroscience
Abstract
A fundamental goal of systems neuroscience is to probe the dynamics of neural activity that drive behavior. Here we present an instrument to simultaneously manipulate neural activity via Channelrhodopsin, monitor neural response via GCaMP3, and observes behavior in freely moving C. elegans. We use the instrument to directly observe the relation between sensory stimuli, interneuron activity and locomotion in the mechanosensory circuit. Now published as: Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028
Keywords
optogenetics, systems neuroscience, calcium imaging, C. elegans, Channelrhodopsin, GCaMP3, neuroscience
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.
DOI of Published Version
10.1101/000943
Source
bioRxiv 000943; doi: https://doi.org/10.1101/000943. Link to preprint on bioRxiv service.
Related Resources
Now published in Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028
Journal/Book/Conference Title
bioRxiv
Repository Citation
Shipley, Frederick B.; Clark, Christopher M.; Alkema, Mark J.; and Leifer, Andrew M., "Simultaneous optogenetic manipulation and calcium imaging in freely moving C. elegans" (2014). University of Massachusetts Medical School Faculty Publications. 1514.
https://escholarship.umassmed.edu/faculty_pubs/1514
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License