Department of Neurobiology; Alkema Lab; Graduate School of Biomedical Sciences, Neuroscience Program
Neuroscience and Neurobiology | Systems Neuroscience
A fundamental goal of systems neuroscience is to probe the dynamics of neural activity that drive behavior. Here we present an instrument to simultaneously manipulate neural activity via Channelrhodopsin, monitor neural response via GCaMP3, and observes behavior in freely moving C. elegans. We use the instrument to directly observe the relation between sensory stimuli, interneuron activity and locomotion in the mechanosensory circuit. Now published as: Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028
optogenetics, systems neuroscience, calcium imaging, C. elegans, Channelrhodopsin, GCaMP3, neuroscience
Rights and Permissions
The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC 4.0 International license.
DOI of Published Version
bioRxiv 000943; doi: https://doi.org/10.1101/000943. Link to preprint on bioRxiv service.
Now published in Front Neural Circuits 8:28, doi:10.3389/fncir.2014.00028
Shipley, Frederick B.; Clark, Christopher M.; Alkema, Mark J.; and Leifer, Andrew M., "Simultaneous optogenetic manipulation and calcium imaging in freely moving C. elegans" (2014). University of Massachusetts Medical School Faculty Publications. 1514.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License