Program in Systems Biology; Program in Molecular Medicine
Developmental Biology | Embryonic Structures | Heterocyclic Compounds | Polycyclic Compounds
Vitamin B12 functions as a cofactor for methionine synthase to produce the anabolic methyl donor S-adenosylmethionine (SAM) and for methylmalonyl-CoA mutase to catabolize the short chain fatty acid propionate. In the nematode Caenorhabditis elegans, maternally supplied vitamin B12 is required for the development of her offspring. However, the mechanism for exporting vitamin B12 from the mother to her offspring is not yet known. Here, we use RNAi of more than 200 transporters with a vitamin B12-sensor transgene to identify the ABC transporter MRP-5 as a candidate vitamin B12 exporter. We show that injection of vitamin B12 into the gonad of mrp-5 deficient mothers rescues embryonic lethality in her offspring. Altogether, our findings identify a maternal mechanism for the transit of an essential vitamin to support the development of the next generation.
C. elegans, multidrug resistance protein 5, vitamin B12, MRP-5, embryo, developmental biology
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The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license.
DOI of Published Version
bioRxiv 204735; doi: https://doi.org/10.1101/204735. Link to preprint on bioRxiv service.
Na H, Ponomarova O, Giese GE, Walhout AJ. (2018). C. elegans Multidrug Resistance Protein 5 (MRP-5) Transports Vitamin B12 from the Intestine to the Gonad to Support Embryonic Development [preprint]. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1101/204735. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1513
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