University of Massachusetts Medical School Faculty Publications

Title

The Vaccine Adjuvant Chitosan Promotes Cellular Immunity via DNA Sensor cGAS-STING-Dependent Induction of Type I Interferons

UMMS Affiliation

Program in Innate Immunity; Division of Infectious Diseases and Immunology, Department of Medicine

Publication Date

2016-03-15

Document Type

Article

Disciplines

Immunology and Infectious Disease

Abstract

The cationic polysaccharide chitosan is an attractive candidate adjuvant capable of driving potent cell-mediated immunity, but the mechanism by which it acts is not clear. We show that chitosan promotes dendritic cell maturation by inducing type I interferons (IFNs) and enhances antigen-specific T helper 1 (Th1) responses in a type I IFN receptor-dependent manner. The induction of type I IFNs, IFN-stimulated genes and dendritic cell maturation by chitosan required the cytoplasmic DNA sensor cGAS and STING, implicating this pathway in dendritic cell activation. Additionally, this process was dependent on mitochondrial reactive oxygen species and the presence of cytoplasmic DNA. Chitosan-mediated enhancement of antigen specific Th1 and immunoglobulin G2c responses following vaccination was dependent on both cGAS and STING. These findings demonstrate that a cationic polymer can engage the STING-cGAS pathway to trigger innate and adaptive immune responses.

DOI of Published Version

10.1016/j.immuni.2016.02.004

Source

Immunity. 2016 Mar 15;44(3):597-608. doi: 10.1016/j.immuni.2016.02.004. Epub 2016 Mar 2. Link to article on publisher's site

Comments

Full author list omitted for brevity. For full list of authors see article.

Related Resources

Link to Article in PubMed

Journal/Book/Conference Title

Immunity

PubMed ID

26944200

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