Program in Gene Function and Expression
Alleles; Animals; Animals, Genetically Modified; Autocrine Communication; Blood Vessels; Cell Movement; Codon, Nonsense; Embryo, Nonmammalian; Female; Lymphatic System; Mice; Mice, Knockout; Neovascularization, Physiologic; Paracrine Communication; Protein Isoforms; Signal Transduction; Vascular Endothelial Growth Factor C; Zebrafish; Zebrafish Proteins
Cellular and Molecular Physiology | Developmental Biology
Vascular endothelial growth factor C (Vegfc) is a secreted protein that guides lymphatic development in vertebrate embryos. However, its role during developmental angiogenesis is not well characterized. Here, we identify a mutation in zebrafish vegfc that severely affects lymphatic development and leads to angiogenesis defects on sensitized genetic backgrounds. The um18 mutation prematurely truncated Vegfc, blocking its secretion and paracrine activity but not its ability to activate its receptor Flt4. When expressed in endothelial cells, vegfc(um18) could not rescue lymphatic defects in mutant embryos, but induced ectopic blood vessel branching. Furthermore, vegfc-deficient endothelial cells did not efficiently contribute to tip cell positions in developing sprouts. Computational modeling together with assessment of endothelial cell dynamics by time-lapse analysis suggested that an autocrine Vegfc/Flt4 loop plays an important role in migratory persistence and filopodia stability during sprouting. Our results suggest that Vegfc acts in two distinct modes during development: as a paracrine factor secreted from arteries to guide closely associated lymphatic vasculature and as an autocrine factor to drive migratory persistence during angiogenesis.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's author rights policy at http://dev.biologists.org/site/misc/rights_permissions.xhtml#author
DOI of Published Version
Development. 2013 Apr;140(7):1497-506. doi: 10.1242/dev.084152. Epub 2013 Mar 5. Link to article on publisher's site
Development (Cambridge, England)
Villefranc, Jacques A.; Nicoli, Stefania; Bentley, Katie; Jeltsch, Michael; Zarkada, Georgia; Moore, John C.; Gerhardt, Holger; Alitalo, Kari; and Lawson, Nathan D., "A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development" (2013). University of Massachusetts Medical School Faculty Publications. 108.