Identifying Nuclear Matrix-Attached DNA Across the Genome
Department of Cell and Developmental Biology; UMass Metabolic Network
Cell Biology | Cellular and Molecular Physiology | Computational Biology | Genomics | Molecular Genetics
Experimental approaches to define the relationship between gene expression and nuclear matrix attachment regions (MARs) have given contrasting and method-specific results. We have developed a next generation sequencing strategy to identify MARs across the human genome (MAR-Seq). The method is based on crosslinking chromatin to its nuclear matrix attachment sites to minimize changes during biochemical processing. We used this method to compare nuclear matrix organization in MCF-10A mammary epithelial-like cells and MDA-MB-231 breast cancer cells and evaluated the results in the context of global gene expression (array analysis) and positional enrichment of gene-regulatory histone modifications (ChIP-Seq). In the normal-like cells, nuclear matrix-attached DNA was enriched in expressed genes, while in the breast cancer cells, it was enriched in non-expressed genes. In both cell lines, the chromatin modifications that mark transcriptional activation or repression were appropriately associated with gene expression. Using this new MAR-Seq approach, we provide the first genome-wide characterization of nuclear matrix attachment in mammalian cells and reveal that the nuclear matrix-associated genome is highly cell-context dependent.
DOI of Published Version
J Cell Physiol. 2016 Sep 14. doi: 10.1002/jcp.25596. Link to article on publisher's site
Journal of cellular physiology
Dobson J, Hong D, Barutcu R, Wu H, Imbalzano AN, Lian JB, Stein JL, Van Wijnen AJ, Nickerson JA, Stein GS. (2016). Identifying Nuclear Matrix-Attached DNA Across the Genome. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1002/jcp.25596. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1051