Dynamics of dengue virus-specific B cells in the response to dengue virus-1 infections using flow cytometry with labeled virions
Department of Medicine, Division of Infectious Diseases and Immunology
Immunology of Infectious Disease | Virology | Virus Diseases
BACKGROUND: The development of reagents to identify and characterize antigen-specific B cells has been challenging.
METHODS: We recently developed Alexa Fluor-labeled dengue viruses (AF DENV) to characterize antigen-specific B cells in the peripheral blood of DENV-immune individuals.
RESULTS: In this study, we used AF DENV-1 together with AF DENV-2 on PBMC from children in Thailand undergoing acute primary or secondary DENV-1 infections to analyze the phenotypes of antigen-specific B cells that reflected their exposure or clinical diagnosis. DENV serotype-specific and cross-reactive B cells were identified in PBMC from all subjects. Frequencies of AF-DENV+ class switched memory B cells (IgD-CD27+ CD19+ cells) reached up to 8% during acute infection and early convalescence. AF DENV-labeled B cells expressed high levels of CD27 and CD38 during acute infection, characteristic of plasmablasts, and transitioned into memory B cells (CD38-CD27+) at the early convalescent time point. There was higher activation of memory B cells early during acute secondary infection suggesting reactivation from a previous DENV infection.
CONCLUSIONS: AF DENV reveal changes in the phenotype of DENV serotype-specific and cross-reactive B cells during and after natural DENV infection and could be useful in analysis of the response to DENV vaccination.
Alexa Fluor, B cell, Dengue, antigen, flow cytometry
DOI of Published Version
J Infect Dis. 2016 Oct 1;214(7):1001-9. doi: 10.1093/infdis/jiw308. Epub 2016 Jul 20. Link to article on publisher's site
The Journal of infectious diseases
Woda M, Friberg H, Currier JR, Srikiatkhachorn A, Macareo LR, Green S, Jarman RG, Rothman AL, Mathew A. (2016). Dynamics of dengue virus-specific B cells in the response to dengue virus-1 infections using flow cytometry with labeled virions. University of Massachusetts Medical School Faculty Publications. https://doi.org/10.1093/infdis/jiw308. Retrieved from https://escholarship.umassmed.edu/faculty_pubs/1033