University of Massachusetts Medical School Faculty Publications


GW182 controls Drosophila circadian behavior and PDF-receptor signaling

UMMS Affiliation

Department of Neurobiology; Emery Lab

Publication Date


Document Type



Animals; Animals, Genetically Modified; Circadian Rhythm; Drosophila; Drosophila Proteins; Gene Expression Regulation, Developmental; Green Fluorescent Proteins; Locomotion; Male; MicroRNAs; RNA Interference; Receptors, G-Protein-Coupled; Signal Transduction; Temperature; Transcription Factors


Molecular and Cellular Neuroscience | Neuroscience and Neurobiology


The neuropeptide PDF is crucial for Drosophila circadian behavior: it keeps circadian neurons synchronized. Here, we identify GW182 as a key regulator of PDF signaling. Indeed, GW182 downregulation results in phenotypes similar to those of Pdf and Pdf-receptor (Pdfr) mutants. gw182 genetically interacts with Pdfr and cAMP signaling, which is essential for PDFR function. GW182 mediates miRNA-dependent gene silencing through its interaction with AGO1. Consistently, GW182's AGO1 interaction domain is required for GW182's circadian function. Moreover, our results indicate that GW182 modulates PDFR signaling by silencing the expression of the cAMP phosphodiesterase DUNCE. Importantly, this repression is under photic control, and GW182 activity level--which is limiting in circadian neurons--influences the responses of the circadian neural network to light. We propose that GW182's gene silencing activity functions as a rheostat for PDFR signaling and thus profoundly impacts the circadian neural network and its response to environmental inputs.

DOI of Published Version



Neuron. 2013 Apr 10;78(1):152-65. doi: 10.1016/j.neuron.2013.01.035. Link to article on publisher's site

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