UMMS Affiliation
Program in Molecular Medicine
Publication Date
2010-05-01
Document Type
Article
Disciplines
Biochemistry | Cell Biology | Cellular and Molecular Physiology | Molecular Biology
Abstract
Neuronal outgrowth occurs via coordinated remodeling of the cytoskeleton involving both actin and microtubules. Microtubule stabilization drives the extending neurite, yet little is known of the molecular mechanisms whereby extracellular cues regulate microtubule dynamics. Bone morphogenetic proteins (BMPs) play an important role in neuronal differentiation and morphogenesis, and BMP7 in particular induces the formation of dendrites. Here, we show that BMP7 induces stabilization of microtubules in both a MAP2-dependent neuronal cell culture model and in dendrites of primary cortical neurons. BMP7 rapidly activates c-Jun N-terminal kinases (JNKs), known regulators of microtubule dynamics, and we show that JNKs associate with the carboxy terminus of the BMP receptor, BMPRII. Activation and binding of JNKs to BMPRII is required for BMP7-induced microtubule stabilization and for BMP7-mediated dendrite formation in primary cortical neurons. These data indicate that BMPRII acts as a scaffold to localize and coordinate cytoskeletal remodeling and thereby provides an efficient means for extracellular cues, such as BMPs, to control neuronal dendritogenesis.
Rights and Permissions
Publisher PDF posted as allowed by the publisher's author rights policy at http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml.
DOI of Published Version
10.1128/MCB.01166-09
Source
Mol Cell Biol. 2010 May;30(9):2241-50. doi: 10.1128/MCB.01166-09. Epub 2010 Feb 22. Link to article on publisher's site
Journal/Book/Conference Title
Molecular and cellular biology
Related Resources
PubMed ID
20176805
Repository Citation
Podkowa, Monika; Zhao, Xin; Chow, Chi-Wing; Coffey, Eleanor T.; Davis, Roger J.; and Attisano, Liliana, "Microtubule stabilization by bone morphogenetic protein receptor-mediated scaffolding of c-Jun N-terminal kinase promotes dendrite formation" (2010). Davis Lab Publications. 84.
https://escholarship.umassmed.edu/davis/84
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