UMMS Affiliation

Program in Molecular Medicine

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Document Type



Biochemistry | Cell Biology | Cellular and Molecular Physiology | Molecular Biology


Bcl2-modifying factor (Bmf) is a member of the BH3-only group of proapoptotic proteins. To test the role of Bmf in vivo, we constructed mice with a series of mutated Bmf alleles that disrupt Bmf expression, prevent Bmf phosphorylation by the c-Jun NH(2)-terminal kinase (JNK) on Ser(74), or mimic Bmf phosphorylation on Ser(74). We report that the loss of Bmf causes defects in uterovaginal development, including an imperforate vagina and hydrometrocolpos. We also show that the phosphorylation of Bmf on Ser(74) can contribute to a moderate increase in levels of Bmf activity. Studies of compound mutants with the related gene Bim demonstrated that Bim and Bmf exhibit partially redundant functions in vivo. Thus, developmental ablation of interdigital webbing on mouse paws and normal lymphocyte homeostasis require the cooperative activity of Bim and Bmf.

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DOI of Published Version



Mol Cell Biol. 2010 Jan;30(1):98-105. doi: 10.1128/MCB.01155-09. Link to article on publisher's site

Journal/Book/Conference Title

Molecular and cellular biology

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PubMed ID