Craig Lab Publications


Structure, sarcomeric organization, and thin filament binding of cardiac myosin-binding protein-C

UMMS Affiliation

Department of Cell and Developmental Biology

Publication Date


Document Type



Biophysics | Cell Biology | Cellular and Molecular Physiology


Myosin-binding protein-C (MyBP-C) is an accessory protein of the myosin filaments of vertebrate striated muscle. In the heart, it plays a key role in modulating contractility in response to beta-adrenergic stimulation. Mutations in the cardiac isoform (cMyBP-C) are a leading cause of inherited hypertrophic cardiomyopathy. Understanding cMyBP-C function and its role in disease requires knowledge of the structure of the molecule, its organization in the sarcomere, and its interactions with other sarcomeric proteins. Here we review the main structural features of this modular, elongated molecule and the properties of some of its key domains. We describe observations suggesting that the bulk of the molecule extends perpendicular to the thick filament, enabling it to reach neighboring thin filaments in the sarcomere. We review structural and functional evidence for interaction of its N-terminal domains with actin and how this may modulate thin filament activation. We also discuss the effects that phosphorylation of cMyBP-C has on some of these structural features and how this might relate to cMyBP-C function in the beating heart.

DOI of Published Version



Craig R, Lee KH, Mun JY, Torre I, Luther PK. Structure, sarcomeric organization, and thin filament binding of cardiac myosin-binding protein-C. Pflugers Arch. 2014 Mar;466(3):425-31. doi: 10.1007/s00424-013-1426-6. Link to article on publisher's site

Journal/Book/Conference Title

Pflugers Archiv : European journal of physiology

Related Resources

Link to Article in PubMed

PubMed ID