Isolation, electron microscopy and 3D reconstruction of invertebrate muscle myofilaments
Department of Cell Biology
Muscle Contraction; Myofibrils; Invertebrates; Microscopy, Electron; Image Processing, Computer-Assisted
Understanding the molecular mechanism of muscle contraction and its regulation has been greatly influenced and aided by studies of myofilament structure in invertebrate muscles. Invertebrates are easily obtained and cover a broad spectrum of species and functional specializations. The thick (myosin-containing) filaments from some invertebrates are especially stable and simple in structure and thus much more amenable to structural analysis than those of vertebrates. Comparative studies of invertebrate filaments by electron microscopy and image processing have provided important generalizations of muscle molecular structure and function. This article reviews methods for preparing thick and thin filaments from invertebrate muscle, for imaging filaments by electron microscopy, and for determining their three dimensional structure by image processing. It also highlights some of the key insights into filament function that have come from these studies.
DOI of Published Version
Methods. 2012 Jan;56(1):33-43. Epub 2011 Dec 2. Link to article on publisher's site
Methods (San Diego, Calif.)
Craig RW. (2012). Isolation, electron microscopy and 3D reconstruction of invertebrate muscle myofilaments. Craig Lab Publications. https://doi.org/10.1016/j.ymeth.2011.11.007. Retrieved from https://escholarship.umassmed.edu/craig/20