Craig Lab Publications


Structure and function of myosin filaments

UMMS Affiliation

Department of Cell Biology



Document Type


Medical Subject Headings

Animals; Microfilaments; Models, Biological; Muscle Contraction; Muscle, Skeletal; Muscle, Smooth; Myosin Subfragments; Myosins


Cell Biology


Myosin filaments interact with actin to generate muscle contraction and many forms of cell motility. X-ray and electron microscopy (EM) studies have revealed the general organization of myosin molecules in relaxed filaments, but technical difficulties have prevented a detailed description. Recent studies using improved ultrastructural and image analysis techniques are overcoming these problems. Three-dimensional reconstructions using single-particle methods have provided many new insights into the organization of the myosin heads and tails. Docking of atomic structures into cryo-EM density maps suggests how regulated myosin filaments are 'switched off', bringing about muscle relaxation. Additionally, sequence analysis suggests probable interactions between myosin tails in the backbone, whereas crystallographic and EM studies are starting to reveal tail interactions directly in three dimensions.

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Citation: Curr Opin Struct Biol. 2006 Apr;16(2):204-12. Epub 2006 Mar 24. Link to article on publisher's site

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Link to Article in PubMed

PubMed ID