Title
Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets
UMMS Affiliation
Department of Cell Biology and Cancer Center
Publication Date
2010-06-22
Document Type
Article
Subjects
Immunoprecipitation; Ribonucleoproteins; MicroRNAs
Disciplines
Cell Biology
Abstract
We have developed a novel Ribonucleoprotein Immunoprecipitation (RNP-IP) method to isolate miR-RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR-RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3'UTR derived primers. This flexible IP-based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time.
DOI of Published Version
10.1002/jcb.22562
Source
J Cell Biochem. 2010 Jul 1;110(4):817-22. Link to article on publisher's site
Journal/Book/Conference Title
Journal of cellular biochemistry
Related Resources
PubMed ID
20564179
Repository Citation
Hassan, Mohammad Q.; Gordon, Jonathan A. R.; Lian, Jane B.; Van Wijnen, Andre J.; Stein, Janet L.; and Stein, Gary S., "Ribonucleoprotein immunoprecipitation (RNP-IP): a direct in vivo analysis of microRNA-targets" (2010). Cell and Developmental Biology Publications. 92.
https://escholarship.umassmed.edu/cellbiology_pp/92