The histone gene cell cycle regulator HiNF-P is a unique zinc finger transcription factor with a novel conserved auxiliary DNA-binding motif
Department of Cell Biology
Amino Acid Motifs; Amino Acid Sequence; Base Sequence; Binding Sites; Cell Cycle; Cell Cycle Proteins; Cell Line; Conserved Sequence; DNA; Hela Cells; Histones; Humans; Models, Molecular; Molecular Sequence Data; Multiprotein Complexes; Mutagenesis; Nuclear Proteins; RNA, Small Interfering; Recombinant Proteins; Repressor Proteins; Sequence Homology, Amino Acid; Zinc Fingers
Accumulation of histone proteins is necessary for packaging of replicated DNA during the S phase of the cell cycle. Different mechanisms operate to regulate histone protein levels, and induction of human histone gene expression at the G1-S phase transition plays a critical role. The zinc finger HiNF-P and coactivator p220 (NPAT) proteins are key regulators of histone gene expression. Here, we describe a novel HiNF-P-specific conserved region (PSCR) located within the C-terminus that is present in HiNF-P homologues of all metazoan species that have been examined. The PSCR motif is required for activation of histone H4 gene transcription and contributes to DNA binding of HiNF-P. Thus, the PSCR module represents an auxiliary DNA-binding determinant that plays a critical role in mediating histone gene expression during the cell cycle and defines HiNF-P as a unique cell cycle regulatory member of the zinc finger transcription factor family.
DOI of Published Version
Biochemistry. 2008 Nov 4;47(44):11415-23. Epub 2008 Oct 14. Link to article on publisher's site
Medina RF, Buck TF, Zaidi SK, Miele A, Lian JB, Stein JL, Van Wijnen AJ, Stein GS. (2008). The histone gene cell cycle regulator HiNF-P is a unique zinc finger transcription factor with a novel conserved auxiliary DNA-binding motif. Cell and Developmental Biology Publications. https://doi.org/10.1021/bi800961d. Retrieved from https://escholarship.umassmed.edu/cellbiology_pp/82