Purification of calmodulin from Chlamydomonas: calmodulin occurs in cell bodies and flagella

UMMS Affiliation

Department of Cell Biology

Publication Date


Document Type



2',3'-Cyclic-Nucleotide Phosphodiesterases; Calcium; Calcium-Binding Proteins; Calmodulin; Chlamydomonas; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Flagella; Heat


Amino Acids, Peptides, and Proteins | Cell Biology | Cells | Investigative Techniques


Calmodulin has been purified from cell bodies of the green alga Chlamydomonas by Ca++-dependent affinity chromatography on fluphenazine-Sepharose 4B. Calmodulin from this primitive organism closely resembles that from bovine brain in a number of properties, including (a) binding to fluphenazine in a Ca++-dependent, reversible manner, (b) functioning as a heat-stable, Ca++-dependent activator of cyclic nucleotide phosphodiesterase, and (c) electrophoretic mobility in SDS-polyacrylamide gels in both the presence and absence of Ca++, which causes a shift in the relative mobility of calmodulin. Calmodulin has also been identified by the criteria of phosphodiesterase activation and electrophoretic mobility in both the detergent soluble "membrane plus matrix" and the axoneme fractions of Chlamydomonas flagella. Calmodulin is not associated with the partially purified 12S or 18S dynein ATPases of Chlamydomonas. The presence of calmodulin in the flagellum suggests that it is involved in one or more of the Ca++-dependent activities of this organelle.

DOI of Published Version



J Cell Biol. 1980 Dec;87(3 Pt 1):764-70.

Journal/Book/Conference Title

The Journal of cell biology

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PubMed ID