Tubulin requires tau for growth onto microtubule initiating sites
Department of Cell Biology
Animals; Flagella; Glycoproteins; Kinetics; Microscopy, Electron; Microtubules; Molecular Weight; Proteins; Tubulin
Amino Acids, Peptides, and Proteins | Cell Biology | Investigative Techniques | Macromolecular Substances
Tubulin purified by phosphocellulose chromatography and free of accessory proteins will not form microtubules in the absence or presence of microtubule initiating sites (flagellar microtubules). The capacity for growth onto pre-existing "seeds" can be restored by the addition of small quantities of partially purified tau protein. Larger quantities restore the capacity for spontaneous assembly. These results suggest that tubulin requires tau for both initiation and growth of microtubules and that tau is incorporated into the microtubule throughout its length.
DOI of Published Version
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4070-4.
Proceedings of the National Academy of Sciences of the United States of America
Witman GB, Cleveland DW, Weingarten MD, Kirschner MW. (1976). Tubulin requires tau for growth onto microtubule initiating sites. Cell and Developmental Biology Publications. https://doi.org/10.1073/pnas.73.11.4070. Retrieved from https://escholarship.umassmed.edu/cellbiology_pp/44