Title
Directionality of brain microtubule assembly in vitro
UMMS Affiliation
Department of Cell Biology
Publication Date
1974-05-01
Document Type
Article
Subjects
Animals; Autoradiography; Brain; Brain Chemistry; Chickens; Electrophoresis, Polyacrylamide Gel; Leucine; Microscopy, Electron; Microtubules; Staining and Labeling; Tritium; Viscosity
Disciplines
Cell Biology
Abstract
Chicks were injected intra-cerebrally with [(3)H]leucine, and (3)H-labeled microtubules were assembled in vitro in the brain supernatants. Pieces of these (3)H-labeled microtubules were then incubated with unlabeled brain tubulin subunits under conditions where the subunits assembled onto the labeled pieces. Electron microscopic autoradiography of the negatively-stained microtubules showed all of the radioactivity at one end of the tubules as they increased in length. This clearly demonstrated that the microtubules of brain in this in vitro system were being assembled unidirectionally.
Source
Proc Natl Acad Sci U S A. 1974 May;71(5):1710-4.
Journal/Book/Conference Title
Proceedings of the National Academy of Sciences of the United States of America
Related Resources
PubMed ID
4134974
Repository Citation
Dentler, William L.; Granett, Sandra; Witman, George B.; and Rosenbaum, Joel L., "Directionality of brain microtubule assembly in vitro" (1974). Cell and Developmental Biology Publications. 42.
https://escholarship.umassmed.edu/cellbiology_pp/42