The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence
Department of Cell Biology; Program in Molecular Medicine; Department of Biochemistry and Molecular Pharmacology
Acetates; Amino Acid Sequence; Animals; Catalysis; Cattle; Cyanogen Bromide; Cyclic AMP-Dependent Protein Kinases; Exons; Introns; Macromolecular Substances; Male; Molecular Sequence Data; Muscle, Skeletal; Myristic Acid; Peptide Fragments; Sequence Alignment; Sheep; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sperm Head; Sperm Tail; Trypsin
Amino Acids, Peptides, and Proteins | Cell Biology | Cells | Enzymes and Coenzymes | Investigative Techniques | Urogenital System
The basis for the unusual properties of the catalytic subunit (C) of ram sperm cAMP-dependent protein kinase was investigated. Ram sperm C was purified and found by mass spectrometry (MS) to be approximately 890 Da smaller than Calpha, the predominant somatic isoform. Partial internal amino acid sequence from ram sperm C was an exact match to that of bovine Calpha, but differed from the predicted sequences for the Cbeta and Cgamma isoforms. MS analysis of 2-nitro-5-thiocyanatobenzoic acid fragments showed that the mass difference originated in the amino-terminal region. A unique blocked amino-terminal fragment was isolated from sperm C and sequenced by a combination of tandem mass spectrometry and Edman degradation of a subfragment. The results revealed that the amino-terminal myristate and the first 14 amino acids of Calpha are replaced by an amino-terminal acetate and six different amino acids in sperm C. The predicted mass difference due to these changes is 899 Da. The region of homology between sperm C and Calpha begins at the exon 1/exon 2 boundary in Calpha, suggesting that sperm C results from use of an alternate exon 1 in the Calpha gene. The different amino terminus of sperm C may be related to a unique requirement for localization of the "free" C subunit within the sperm flagellum.
DOI of Published Version
J Biol Chem. 1998 Sep 18;273(38):24874-83.
The Journal of biological chemistry
San Agustin JT, Leszyk JD, Nuwaysir LM, Witman GB. (1998). The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence. Cell and Developmental Biology Publications. https://doi.org/10.1074/jbc.273.38.24874. Retrieved from https://escholarship.umassmed.edu/cellbiology_pp/22