C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization; UMass Metabolic Network

UMMS Affiliation

Department of Cell and Developmental Biology



Document Type



Three-dimensional organization of the chromatin has important roles in transcription, replication, DNA repair, and pathologic events such as translocations. There are two fundamental ways to study higher-order chromatin organization: microscopic and molecular approaches. In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or "3C" technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques. We further discuss the different types of data generated by the 3C-based methods and how they can be used to answer distinct biological questions.

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Citation: J Cell Physiol. 2016 Jan;231(1):31-5. doi: 10.1002/jcp.25062. Link to article on publisher's site

Related Resources

Link to Article in PubMed

Journal Title

Journal of cellular physiology

PubMed ID