C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization; UMass Metabolic Network
Department of Cell and Developmental Biology
Cell Biology | Cellular and Molecular Physiology | Computational Biology | Genomics | Molecular Biology
Three-dimensional organization of the chromatin has important roles in transcription, replication, DNA repair, and pathologic events such as translocations. There are two fundamental ways to study higher-order chromatin organization: microscopic and molecular approaches. In this review, we briefly introduce the molecular approaches, focusing on chromosome conformation capture or "3C" technology and its derivatives, which can be used to probe chromatin folding at resolutions beyond that provided by microscopy techniques. We further discuss the different types of data generated by the 3C-based methods and how they can be used to answer distinct biological questions.
DOI of Published Version
J Cell Physiol. 2016 Jan;231(1):31-5. doi: 10.1002/jcp.25062. Link to article on publisher's site
Journal of cellular physiology
Barutcu AR, Fritz AJ, Zaidi SK, van Wijnen AJ, Lian JB, Stein JL, Nickerson JA, Imbalzano AN, Stein GS. (2016). C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization; UMass Metabolic Network. Cell and Developmental Biology Publications. https://doi.org/10.1002/jcp.25062. Retrieved from https://escholarship.umassmed.edu/cellbiology_pp/170