Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In Vivo

Charusheila Ramkumar, University of Massachusetts Medical School
Rachel M. Gerstein, University of Massachusetts Medical School
Hong Zhang, University of Massachusetts Medical School

First author Charusheila Ramkumar is a student in the Cell Biology program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

Abstract

Hematopoietic stem cells (HSCs) have the ability to self-renew and replenish the blood and immune system for the life span of an individual. An age-associated decline in HSC function is responsible for the decreased immune function and increased incidence of myeloid diseases and anemia in the elderly. The changes in HSC function are thought to occur as the result of an intrinsic defect in the self-renewal potential of HSCs as they age. In this chapter, we describe a bone marrow serial transplantation protocol designed to test the self-renewal capacity of HSCs in vivo.