Serial Transplantation of Bone Marrow to Test Self-renewal Capacity of Hematopoietic Stem Cells In Vivo

UMMS Affiliation

Department of Cell and Developmental Biology; Department of Microbiology and Physiological Systems

Publication Date


Document Type

Book Chapter


Hematopoietic Stem Cells; Bone Marrow Transplantation


Cancer Biology | Cell and Developmental Biology | Cell Biology


Hematopoietic stem cells (HSCs) have the ability to self-renew and replenish the blood and immune system for the life span of an individual. An age-associated decline in HSC function is responsible for the decreased immune function and increased incidence of myeloid diseases and anemia in the elderly. The changes in HSC function are thought to occur as the result of an intrinsic defect in the self-renewal potential of HSCs as they age. In this chapter, we describe a bone marrow serial transplantation protocol designed to test the self-renewal capacity of HSCs in vivo.

DOI of Published Version



Ramkumar C, Gerstein RM, Zhang H. Serial transplantation of bone marrow to test self-renewal capacity of hematopoietic stem cells in vivo. Methods Mol Biol. 2013;976:17-24. doi: 10.1007/978-1-62703-317-6_2. Link to article on publisher's site

Journal/Book/Conference Title

Methods in molecular biology (Clifton, N.J.)


First author Charusheila Ramkumar is a student in the Cell Biology program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School.

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Link to Article in PubMed

PubMed ID