Title

The BRCA1-associated protein BACH1 is a DNA helicase targeted by clinically relevant inactivating mutations

UMMS Affiliation

Department of Cancer Biology; Program in Molecular Medicine

Publication Date

2004-02-26

Document Type

Article

Subjects

Adenosine Triphosphatases; Amino Acid Substitution; BRCA1 Protein; Basic-Leucine Zipper Transcription Factors; DNA Helicases; Fanconi Anemia Complementation Group Proteins; Germ-Line Mutation; Humans; Leucine Zippers; Mutagenesis, Site-Directed; Nuclear Proteins; Transcription Factors

Disciplines

Amino Acids, Peptides, and Proteins | Cancer Biology | Genetic Phenomena | Neoplasms

Abstract

BACH1 is a nuclear protein that directly interacts with the highly conserved, C-terminal BRCT repeats of the tumor suppressor, BRCA1. Mutations within the BRCT repeats disrupt the interaction between BRCA1 and BACH1, lead to defects in DNA repair, and result in breast and ovarian cancer. BACH1 is necessary for efficient double-strand break repair in a manner that depends on its association with BRCA1. Moreover, some women with early-onset breast cancer and no abnormalities in either BRCA1 or BRCA2 carry germline BACH1 coding sequence changes, suggesting that abnormal BACH1 function contributes to tumor induction. Here, we show that BACH1 is both a DNA-dependent ATPase and a 5'-to-3' DNA helicase. In two patients with early-onset breast cancer who carry distinct germline BACH1 coding sequence changes, the resulting proteins are defective in helicase activity, indicating that these sequence changes disrupt protein function. These results reinforce the notion that mutant BACH1 participates in breast cancer development.

DOI of Published Version

10.1073/pnas.0308717101

Source

Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2357-62.

Journal/Book/Conference Title

Proceedings of the National Academy of Sciences of the United States of America

Related Resources

Link to Article in PubMed

PubMed ID

14983014

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