Carbohydrate-binding protein 35 is the major cell-surface laminin-binding protein in colon carcinoma
Department of Cancer Biology
Antigens, Differentiation; Carcinoma; Carrier Proteins; Chromatography, Affinity; Colonic Neoplasms; Flow Cytometry; Galectin 3; Hemagglutinins; Humans; Immunoblotting; Membrane Proteins; Neoplasm Proteins; Tumor Cells, Cultured
Amino Acids, Peptides, and Proteins | Cancer Biology | Carbohydrates | Cells | Digestive System Diseases | Neoplasms
The interaction of tumor cells with laminin is thought to be critical in invasion and metastasis. We found that an endogenous lectin, carbohydrate-binding protein 35 (CBP-35), is the major laminin-binding protein on human colon carcinoma cells and that its surface expression suggests involvement in metastasis. We identified CBP-35 by laminin-affinity chromatography and immunoblotting. Surface expression of CBP-35 on eight human colon carcinoma cell lines was compared by flow cytometry. Poorly differentiated cell lines and DLD-2, a signet-ring carcinoma cell line, expressed more surface CBP-35 than well-differentiated cell lines. Poorly differentiated cell lines and DLD-2 are characterized as aggressive cell lines because they adhere to and invade through reconstituted basement membrane significantly better than well-differentiated cell lines. These data suggest that CBP-35 is involved in tumor cell-basement membrane interactions and that an increase in CBP-35 surface expression may facilitate metastatic potential of colon carcinoma cells.
DOI of Published Version
Arch Surg. 1991 Dec;126(12):1498-502.
Archives of surgery (Chicago, Ill. : 1960)
Lee EC, Woo H, Korzelius CA, Steele GD, Mercurio AM. (1991). Carbohydrate-binding protein 35 is the major cell-surface laminin-binding protein in colon carcinoma. Cancer Biology Publications and Presentations. https://doi.org/10.1001/archsurg.1991.01410360072011. Retrieved from https://escholarship.umassmed.edu/cancerbiology_pp/120