Title
ATP-Dependent Sugar Transport Complexity in Human Erythrocytes
UMMS Affiliation
Graduate School of Biomedical Sciences; Department of Biochemistry and Molecular Pharmacology
Publication Date
2007-02-13
Document Type
Article
Subjects
3-O-Methylglucose; Adenosine Triphosphate; Biological Transport; Cell Size; Cytoplasm; Equilibrative Nucleoside Transporter 1; Erythrocyte Membrane; Erythrocytes; Glucose; Glucose Transporter Type 1; Humans; Microscopy, Electron, Scanning; Models, Biological; Uridine
Disciplines
Medical Biochemistry | Medical Biophysics | Physiological Processes
Abstract
Human erythrocyte glucose sugar transport was examined in resealed red cell ghosts under equilibrium exchange conditions ([sugar](intracellular) = [sugar](extracellular), where brackets indicate concentration). Exchange 3-O-methylglucose (3MG) import and export are monophasic in the absence of cytoplasmic ATP but are biphasic when ATP is present. Biphasic exchange is observed as the rapid filling of a large compartment (66% cell volume) followed by the slow filling of the remaining cytoplasmic space. Biphasic exchange at 20 mM 3MG eliminates the possibility that the rapid exchange phase represents ATP-dependent 3MG binding to the glucose transport protein (GLUT1; cellular [GLUT1] of
Keywords
Membrane transport; erythrocytes; GLUT1
DOI of Published Version
10.1152/ajpcell.00335.2006
Source
Am J Physiol Cell Physiol. 2007 Feb;292(2):C974-86. Epub 2006 Aug 23. Link to article on publisher's site
Journal/Book/Conference Title
American journal of physiology. Cell physiology
Related Resources
PubMed ID
16928769
Repository Citation
Leitch, Jeffry M. and Carruthers, Anthony, "ATP-Dependent Sugar Transport Complexity in Human Erythrocytes" (2007). Biochemistry and Molecular Pharmacology Publications and Presentations. 61.
https://escholarship.umassmed.edu/bmp_pp/61