Department of Biochemistry and Molecular Pharmacology
Biochemistry, Biophysics, and Structural Biology | Pharmacology, Toxicology and Environmental Health
BACKGROUND: Initiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations.
METHODOLOGY/PRINCIPAL FINDINGS: DNA microarrays were used to measure mRNA steady-state levels in initiation-deficient dnaA46 and dnaC2 bacteria at permissive and non-permissive temperatures and their expression profiles were compared to MG1655 wildtype cells. For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature. Transcription of the dnaA and dnaC genes was increased at the non-permissive temperature in the respective mutant strains indicating auto-regulation of both genes. Induction of the SOS regulon was observed in dnaC2 cells at 38 degrees C and 42 degrees C. Flow cytometric analysis revealed that dnaC2 mutant cells at non-permissive temperature had completed the early stages of chromosome replication initiation.
CONCLUSION/SIGNIFICANCE: We suggest that in dnaC2 cells the SOS response is triggered by persistent open-complex formation at oriC and/or by arrested forks that require DnaC for replication restart.
DOI of Published Version
PLoS ONE. 2008 Aug 20;3(8):e2984. Link to article on publisher's site
Lobner-Olesen A, Slominska-Wojewodzka M, Hansen FG, Marinus MG. (2008). DnaC inactivation in Escherichia coli K-12 induces the SOS response and expression of nucleotide biosynthesis genes. Biochemistry and Molecular Pharmacology Publications. https://doi.org/10.1371/journal.pone.0002984. Retrieved from https://escholarship.umassmed.edu/bmp_pp/55