Title

Growth-rate-dependent transcription initiation from the dam P2 promoter

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

1995-05-19

Document Type

Article

Subjects

Base Sequence; Colon; Escherichia coli; Escherichia coli Proteins; *Gene Expression Regulation, Bacterial; Humans; Methyltransferases; *Promoter Regions, Genetic; Recombinant Fusion Proteins; *Site-Specific DNA-Methyltransferase (Adenine-Specific); Substrate Specificity; beta-Galactosidase

Disciplines

Bacteria | Biochemistry, Biophysics, and Structural Biology | Genetic Phenomena | Pharmacology, Toxicology and Environmental Health

Abstract

Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.

Keywords

DNA methylation, lipoic acid ligase, recombinant DNA, gene regulation

DOI of Published Version

10.1016/0378-1119(94)00619-4

Source

Gene. 1995 May 19;157(1-2):213-5.

Journal/Book/Conference Title

Gene

Related Resources

Link to Article in PubMed

PubMed ID

7607492

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