Growth-rate-dependent transcription initiation from the dam P2 promoter
Department of Biochemistry and Molecular Pharmacology
Base Sequence; Colon; Escherichia coli; Escherichia coli Proteins; *Gene Expression Regulation, Bacterial; Humans; Methyltransferases; *Promoter Regions, Genetic; Recombinant Fusion Proteins; *Site-Specific DNA-Methyltransferase (Adenine-Specific); Substrate Specificity; beta-Galactosidase
Bacteria | Biochemistry, Biophysics, and Structural Biology | Genetic Phenomena | Pharmacology, Toxicology and Environmental Health
Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.
DNA methylation, lipoic acid ligase, recombinant DNA, gene regulation
DOI of Published Version
Gene. 1995 May 19;157(1-2):213-5.
Rasmussen LJ, Lobner-Olesen A, Marinus MG. (1995). Growth-rate-dependent transcription initiation from the dam P2 promoter. Biochemistry and Molecular Pharmacology Publications. https://doi.org/10.1016/0378-1119(94)00619-4. Retrieved from https://escholarship.umassmed.edu/bmp_pp/45