Expression of the Escherichia coli dam gene
Department of Biochemistry and Molecular Pharmacology
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli; Escherichia coli Proteins; Flow Cytometry; *Gene Expression Regulation, Bacterial; Genes, Bacterial; Methyltransferases; Molecular Sequence Data; Mutation; Operon; Promoter Regions, Genetic; *Site-Specific DNA-Methyltransferase (Adenine-Specific); Transcription Factors
Biochemistry, Biophysics, and Structural Biology | Pharmacology, Toxicology and Environmental Health
The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.
Mol Microbiol. 1992 Jul;6(13):1841-51.
Lobner-Olesen A, Boye E, Marinus MG. (1992). Expression of the Escherichia coli dam gene. Biochemistry and Molecular Biotechnology Publications. Retrieved from https://escholarship.umassmed.edu/bmp_pp/40