Quantitation of Dam methyltransferase in Escherichia coli
Department of Biochemistry and Molecular Pharmacology
Blotting, Western; Escherichia coli; Escherichia coli Proteins; Immunoenzyme Techniques; Methyltransferases; *Site-Specific DNA-Methyltransferase (Adenine-Specific)
Bacteria | Biochemistry, Biophysics, and Structural Biology | Pharmacology, Toxicology and Environmental Health
An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.
DOI of Published Version
J Bacteriol. 1992 Mar;174(5):1682-5.
Journal of bacteriology
Boye E, Marinus MG, Lobner-Olesen A. (1992). Quantitation of Dam methyltransferase in Escherichia coli. Biochemistry and Molecular Pharmacology Publications. https://doi.org/10.1128/jb.174.5.1682-1685.1992. Retrieved from https://escholarship.umassmed.edu/bmp_pp/39