UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

2018-03-23

Document Type

Article

Disciplines

Amino Acids, Peptides, and Proteins | Biochemistry | Cellular and Molecular Physiology | Chemistry | Molecular Biology

Abstract

Voltage-gated Ca(2+) (CaV ) channels mediate Ca(2+) entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo-tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications in interrogating a wide range of CaV -mediated physiological processes.

Keywords

calcium signaling, ion channels, optogenetics, protein engineering, synthetic biology

Rights and Permissions

© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

DOI of Published Version

10.1002/anie.201713080

Source

Angew Chem Int Ed Engl. 2018 Mar 23. doi: 10.1002/anie.201713080. Link to article on publisher's site

Journal/Book/Conference Title

Angewandte Chemie (International ed. in English)

Related Resources

Link to Article in PubMed

PubMed ID

29569306

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

Share

COinS