Title

Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute

Publication Date

2016-05-01

Document Type

Article

Disciplines

Biochemistry | Biotechnology | Cell Biology | Genomics | Molecular Biology | Molecular, Cellular, and Tissue Engineering | Structural Biology

Abstract

A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of fluorescent proteins. We demonstrate simultaneous imaging of up to six chromosomal loci in individual live cells and document large differences in the dynamic properties of different chromosomal loci.

Keywords

Cellular imaging, Chromosomes, Fluorescence imaging, Molecular engineering, RNA

DOI of Published Version

10.1038/nbt.3526

Source

Nat Biotechnol. 2016 May;34(5):528-30. doi: 10.1038/nbt.3526. Epub 2016 Apr 18. Link to article on publisher's website

Journal/Book/Conference Title

Nature biotechnology

Related Resources

Link to article in PubMed

PubMed ID

27088723

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