Title
Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
1983-01-01
Document Type
Article
Subjects
Cloning, Molecular; DNA (Cytosine-5-)-Methyltransferase; DNA, Recombinant; Escherichia coli; *Genes, Bacterial; Methyltransferases; Mutation; Phenotype; Plasmids; Site-Specific DNA-Methyltransferase (Adenine-Specific); *Transformation, Bacterial
Disciplines
Biochemistry, Biophysics, and Structural Biology
Abstract
A recombinant plasmid, pMQ3, carrying the dam gene of Escherichia coli K-12, was constructed and transformed into dam+ and dam- strains. Both dam- and dam+ strains containing pMQ3 showed a wild phenotype for all traits, including mutation rate, except for a 10-fold increase in DNA adenine methylase activity.
Source
J Bacteriol. 1983 Jan;153(1):562-5.
Journal/Book/Conference Title
Journal of bacteriology
Related Resources
PubMed ID
6336742
Repository Citation
Arraj JA, Marinus MG. (1983). Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene. Biochemistry and Molecular Biotechnology Publications. Retrieved from https://escholarship.umassmed.edu/bmp_pp/18