Cell biology of mRNA decay
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Animals; Cell Survival; Humans; In Situ Hybridization, Fluorescence; Microscopy; Proteins; RNA Stability; RNA, Messenger
Biochemistry | Biochemistry, Biophysics, and Structural Biology | Cell Biology | Molecular Biology | Molecular Genetics | Structural Biology
Studying single mRNA molecules has added new dimensions to our understanding of gene expression and the life cycle of mRNA in cells. Advances in microscopes and detection technology have opened access to single molecule research to most researchers interested in molecular biology. Here we provide an overview technique for single molecule studies of RNA in either fixed samples or in living cells. As part of a volume on mRNA turnover, it is increasingly relevant, because many of the recent advances in studies of mRNA turnover have suggested that there is non-homogeneous distribution of turnover factors in the cell. For this reason, understanding of spatial relationships between mRNA and mRNA turnover factors should enrich our understanding of this process.
DOI of Published Version
Grünwald D, Singer RH, Czaplinski K. Cell biology of mRNA decay. Methods Enzymol. 2008;448:553-77. doi: 10.1016/S0076-6879(08)02627-X. Link to article on publisher's site
Methods in enzymology
Grunwald, David; Singer, Robert H.; and Czaplinski, Kevin, "Cell biology of mRNA decay" (2008). Biochemistry and Molecular Pharmacology Publications and Presentations. 179.