Title

Direct observation of single protein molecules in aqueous solution

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute

Publication Date

2006-04-10

Document Type

Article

Subjects

Carbocyanines; Diffusion; Microscopy, Fluorescence; Microscopy, Video; Sensitivity and Specificity; Solutions; Streptavidin; Time Factors; Water

Disciplines

Biochemistry | Biochemistry, Biophysics, and Structural Biology | Biological and Chemical Physics | Chemistry | Physical Chemistry

Abstract

Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.

DOI of Published Version

10.1002/cphc.200500632

Source

Grünwald, D., Hoekstra, A., Dange, T., Buschmann, V. and Kubitscheck, U. (2006), Direct Observation of Single Protein Molecules in Aqueous Solution. ChemPhysChem, 7: 812–815. doi: 10.1002/cphc.200500632

Journal/Book/Conference Title

Chemphyschem : a European journal of chemical physics and physical chemistry

Comments

At the time of publication, David Grünwald was not yet affiliated with the University of Massachusetts Medical School.

Related Resources

Link to Article in PubMed

PubMed ID

16528778

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