Direct observation of single protein molecules in aqueous solution
Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute
Carbocyanines; Diffusion; Microscopy, Fluorescence; Microscopy, Video; Sensitivity and Specificity; Solutions; Streptavidin; Time Factors; Water
Biochemistry | Biochemistry, Biophysics, and Structural Biology | Biological and Chemical Physics | Chemistry | Physical Chemistry
Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.
DOI of Published Version
Grünwald, D., Hoekstra, A., Dange, T., Buschmann, V. and Kubitscheck, U. (2006), Direct Observation of Single Protein Molecules in Aqueous Solution. ChemPhysChem, 7: 812–815. doi: 10.1002/cphc.200500632
Chemphyschem : a European journal of chemical physics and physical chemistry
Grunwald, David; Hoekstra, Andreas; Dange, Thomas; Buschmann, Volker; and Kubitscheck, Ulrich, "Direct observation of single protein molecules in aqueous solution" (2006). Biochemistry and Molecular Pharmacology Publications and Presentations. 176.