Direct observation of single protein molecules in aqueous solution

David Grunwald, University of Massachusetts Medical School
Andreas Hoekstra, Rheinische Friedrich-Wilhelms-Universitat
Thomas Dange, Rheinische Friedrich-Wilhelms-Universitat
Volker Buschmann, Max-Delbruck-Centrum
Ulrich Kubitscheck, Rheinische Friedrich-Wilhelms-Universitat

At the time of publication, David Grünwald was not yet affiliated with the University of Massachusetts Medical School.


Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.