Direct observation of single protein molecules in aqueous solution

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; RNA Therapeutics Institute



Document Type


Medical Subject Headings

Carbocyanines; Diffusion; Microscopy, Fluorescence; Microscopy, Video; Sensitivity and Specificity; Solutions; Streptavidin; Time Factors; Water


Single molecule tracking (SMT) by fluorescence video microscopy has matured to be a well established method in the last few years. In this report we demonstrate the imaging and tracking of single protein molecules in a phosphate/Hepes buffer with high sensitivity at frame rates of 350 Hz.


Citation: Grünwald, D., Hoekstra, A., Dange, T., Buschmann, V. and Kubitscheck, U. (2006), Direct Observation of Single Protein Molecules in Aqueous Solution. ChemPhysChem, 7: 812–815. doi: 10.1002/cphc.200500632

At the time of publication, David Grünwald was not yet affiliated with the University of Massachusetts Medical School.

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Link to Article in PubMed

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