Characterization of DNA adenine methylation mutants of Escherichia coli K12
Department of Biochemistry and Molecular Pharmacology
Adenine; Conjugation, Genetic; DNA (Cytosine-5-)-Methyltransferase; DNA, Bacterial; Escherichia coli; Genotype; Methyltransferases; *Mutation; Phenotype; Recombination, Genetic; Transduction, Genetic
Biochemistry, Biophysics, and Structural Biology
The phenotypic traits of 7 independently isolated dam mutants of Escherichia coli have been examined. The mutant strains differ from the wildtype in the following respects: (1) decreased DNA adenine methylase activity in vivo and in vitro; (2) a 14--85-fold increase in spontaneous mutability; (3) decreased survival after ultraviolet irradiation; (4) a 10--21-fold increase in spontaneous induction of lambda phage from lysogens; (5) a 3--17-fold increase in the level of recombination; and (6) inviability of double mutants containing dam- and recB- or recC-. Unmethylated fd phage chromosomes are able to replicate normally in dam- mutants. A mutant strain in which the dcm gene is deleted is viable, showing that the dcm gene product is dispensible for growth.
Mutat Res. 1979 Feb;59(2):157-65.
Bale A, d'Alarcao M, Marinus MG. (1979). Characterization of DNA adenine methylation mutants of Escherichia coli K12. Biochemistry and Molecular Pharmacology Publications. Retrieved from https://escholarship.umassmed.edu/bmp_pp/14