Title
Hyper-recombination in dam mutants of Escherichia coli K-12
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology
Publication Date
1976-12-22
Document Type
Article
Subjects
Chromosome Mapping; Chromosomes, Bacterial; DNA (Cytosine-5-)-Methyltransferase; Escherichia coli; Genotype; *Mutation; *Recombination, Genetic; Species Specificity
Disciplines
Biochemistry, Biophysics, and Structural Biology
Abstract
F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30-200 times higher than the isogenic dam+ strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduction in linkage of proximal unselected markers in transconjugants and increases the recombination frequency between a pair of closely linked markers. It is concluded that dam mutations confer a hyperrecombination phenotype to the cell.
Source
Mol Gen Genet. 1976 Dec 22;149(3):273-7.
Journal/Book/Conference Title
Molecular and general genetics : MGG
Related Resources
PubMed ID
799245
Repository Citation
Marinus, Martin G. and Konrad, E. Bruce, "Hyper-recombination in dam mutants of Escherichia coli K-12" (1976). Biochemistry and Molecular Pharmacology Publications and Presentations. 13.
https://escholarship.umassmed.edu/bmp_pp/13