Hyper-recombination in dam mutants of Escherichia coli K-12

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology



Document Type


Medical Subject Headings

Chromosome Mapping; Chromosomes, Bacterial; DNA (Cytosine-5-)-Methyltransferase; Escherichia coli; Genotype; *Mutation; *Recombination, Genetic; Species Specificity


F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30-200 times higher than the isogenic dam+ strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduction in linkage of proximal unselected markers in transconjugants and increases the recombination frequency between a pair of closely linked markers. It is concluded that dam mutations confer a hyperrecombination phenotype to the cell.

Rights and Permissions

Citation: Mol Gen Genet. 1976 Dec 22;149(3):273-7.

Related Resources

Link to Article in PubMed

PubMed ID