Removal of PEG and salt from recombinant human tumor necrosis factor-alpha(rhTNF-alpha) with serial operation of gel filtration chromatography
Department of Biochemistry and Molecular Pharmacology
Chromatography, Gel; Polyethylene Glycols; Recombinant Proteins; Sodium Chloride; Tumor Necrosis Factor-alpha
Biochemistry, Biophysics, and Structural Biology | Pharmacology, Toxicology and Environmental Health
A serial gel filtration operation with the combination of G25 and G100 in one column was developed to remove salt and PEG from upper phase rich in recombinant human tumor necrosis factor alpha after simultaneous cell disruption and two-phase aqueous extraction. Buffer exchanging and primary purification were also achieved at same time. The purification factor of 4.4 was obtained with an activity recovery of 97% by one single step of serial operation.
Sheng Wu Gong Cheng Xue Bao. 2001 Jul;17(4):471-3.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Gu, Zhenyu; Feng, X. L.; and Su, Zhiguo, "Removal of PEG and salt from recombinant human tumor necrosis factor-alpha(rhTNF-alpha) with serial operation of gel filtration chromatography" (2001). Biochemistry and Molecular Pharmacology Publications and Presentations. 122.