Title

Removal of PEG and salt from recombinant human tumor necrosis factor-alpha(rhTNF-alpha) with serial operation of gel filtration chromatography

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

2001-11-13

Document Type

Article

Subjects

Chromatography, Gel; Polyethylene Glycols; Recombinant Proteins; Sodium Chloride; Tumor Necrosis Factor-alpha

Disciplines

Biochemistry, Biophysics, and Structural Biology | Pharmacology, Toxicology and Environmental Health

Abstract

A serial gel filtration operation with the combination of G25 and G100 in one column was developed to remove salt and PEG from upper phase rich in recombinant human tumor necrosis factor alpha after simultaneous cell disruption and two-phase aqueous extraction. Buffer exchanging and primary purification were also achieved at same time. The purification factor of 4.4 was obtained with an activity recovery of 97% by one single step of serial operation.

Source

Sheng Wu Gong Cheng Xue Bao. 2001 Jul;17(4):471-3.

Journal/Book/Conference Title

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

Comments

Article is written in Chinese

Related Resources

Link to Article in PubMed

PubMed ID

11702713

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