Title

Preparation of bovine lipid-free hemoglobin

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology

Publication Date

2003-02-04

Document Type

Article

Subjects

Animals; Cattle; Hemoglobins; Hydrophobicity; Lipids; Polyethylene Glycols

Disciplines

Biochemistry, Biophysics, and Structural Biology | Pharmacology, Toxicology and Environmental Health

Abstract

A new method for preparation of Hb solution free of stromal lipid was described. Almost all the lipid in fresh hemolysate of bovine red blood was removed with hydrophobic interaction chromatography (HIC) in the presence of 2% PEG4000, 5% PEG4000, 2%PEG10000 or 5%PEG10000. With the adding of 5%PEG4000, the 80% of recovery of Hb in HIC was obtained and the maximum lipid absorbed by hydrophobic medium, butyl agarose -6B was 86.6 mg/mL. The activity (P50) of hemoglobin preparation was 3386.4 Pa torrs, and the Hill number was 2.54, which were near to that of the native red blood cells. The mechanism of removing lipid by HIC and the function of PEG in the process were discussed.

Source

Sheng Wu Gong Cheng Xue Bao. 2002 Sep;18(5):609-13.

Journal/Book/Conference Title

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

Comments

Article is written in Chinese

Related Resources

Link to Article in PubMed

PubMed ID

12561209

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