Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases
Department of Biochemistry and Molecular Pharmacology
Chromatography, Affinity; Chromatography, Thin Layer; Chromosome Mapping; Electrophoresis; Escherichia coli; Genes; Guanosine; Methyltransferases; Mutagens; *Mutation; Nitrosoguanidines; RNA, Bacterial; RNA, Ribosomal; RNA, Transfer; Recombination, Genetic; S-Adenosylmethionine; Thymidine; Uridine
Bacteria | Biochemistry, Biophysics, and Structural Biology | Genetic Phenomena | Nucleic Acids, Nucleotides, and Nucleosides
Seven transfer ribonucleic acid (tRNA) methylase mutants were isolated from Escherichia coli K-12 by examining the ability of RNA prepared from clones of unselected mutagenized cells to accept methyl groups from S-adenosylmethionine catalyzed by crude enzymes from wild-type cells. Five of the mutants had an altered uracil-tRNA methylase; consequently their tRNA's lacked ribothymidine. One mutant had tRNA deficient in 7-methylguanosine, and one mutant contained tRNA lacking 2-thio-5-methylaminomethyluridine. The genetic loci of the three tRNA methylase mutants were distributed over the E. coli genome. The mutant strain deficient in 7-methylguanosine biosynthesis showed a reduced efficiency in the suppression of amber mutations carried by T4 or lambda phages.
J Bacteriol. 1975 Apr;122(1):257-65.
Journal of bacteriology
Marinus MG, Morris NR, Soll D, Kwong TC. (1975). Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. Biochemistry and Molecular Biotechnology Publications. Retrieved from https://escholarship.umassmed.edu/bmp_pp/10