Mapping Billions of Short Reads to a Reference Genome
Program in Bioinformatics and Integrative Biology; Department of Biochemistry and Molecular Pharmacology
Bioinformatics | Computational Biology | Genomics | Molecular Biology
Rapid development and commercialization of instruments that can accurately, rapidly, and cheaply sequence billions of DNA bases is revolutionizing molecular biology and medicine. Because a reference genome is usually available, the first bioinformatics challenge presented by the new generation of high-throughput sequencers is the genome mapping problem, where each read is mapped to a reference genome to reveal its location(s). An introduction to mapping algorithms, as well as factors that influence their results, is provided here.
DOI of Published Version
Cold Spring Harb Protoc. 2017 Jan 3;2017(1):pdb.top093153. doi: 10.1101/pdb.top093153. Link to article on publisher's site
Cold Spring Harbor protocols
Hung, Jui-Hung and Weng, Zhiping, "Mapping Billions of Short Reads to a Reference Genome" (2016). Program in Bioinformatics and Integrative Biology Publications and Presentations. 90.