Simultaneous generation of many RNA-seq libraries in a single reaction
Program in Bioinformatics and Integrative Biology; Bioinformatics Core
Bacteria; *Base Sequence; Gene Expression Profiling; *Gene Library; Sequence Analysis, RNA; Time Factors
Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.
DOI of Published Version
Nat Methods. 2015 Apr;12(4):323-5. doi: 10.1038/nmeth.3313. Epub 2015 Mar 2. Link to article on publisher's site
Shishkin AA, Giannoukos G, Kucukural A, Ciulla D, Busby M, Surka C, Chen J, Bhattacharyya RP, Rudy RF, Patel MM, Novod N, Hung DT, Gnirke A, Garber M, Guttman M, Livny J. (2015). Simultaneous generation of many RNA-seq libraries in a single reaction. Program in Bioinformatics and Integrative Biology Publications. https://doi.org/10.1038/nmeth.3313. Retrieved from https://escholarship.umassmed.edu/bioinformatics_pubs/80