Simultaneous generation of many RNA-seq libraries in a single reaction
Program in Bioinformatics and Integrative Biology; Bioinformatics Core
Bacteria; *Base Sequence; Gene Expression Profiling; *Gene Library; Sequence Analysis, RNA; Time Factors
Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Computational Biology | Integrative Biology | Systems Biology
Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.
DOI of Published Version
Nat Methods. 2015 Apr;12(4):323-5. doi: 10.1038/nmeth.3313. Epub 2015 Mar 2. Link to article on publisher's site
Shishkin, Alexander A.; Giannoukos, Georgia; Kucukural, Alper; Ciulla, Dawn; Busby, Michele; Surka, Christine; Chen, Jenny; Bhattacharyya, Roby P.; Rudy, Robert F.; Patel, Milesh M.; Novod, Nathaniel; Hung, Deborah T.; Gnirke, Andreas; Garber, Manuel; Guttman, Mitchell; and Livny, Jonathan, "Simultaneous generation of many RNA-seq libraries in a single reaction" (2015). Program in Bioinformatics and Integrative Biology Publications and Presentations. 80.