Dicer partner proteins tune the length of mature miRNAs in flies and mammals
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UMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyDepartment of Biochemistry and Molecular Pharmacology
Document Type
Journal ArticlePublication Date
2012-10-26Keywords
AnimalsBase Sequence
DEAD-box RNA Helicases
Drosophila Proteins
Drosophila melanogaster
Female
Humans
Male
Mice
MicroRNAs
Molecular Sequence Data
RNA Helicases
RNA-Binding Proteins
Ribonuclease III
Biochemistry, Biophysics, and Structural Biology
Bioinformatics
Molecular Biology
Metadata
Show full item recordAbstract
Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. Alternative splicing of the loquacious (loqs) mRNA generates three distinct Dicer partner proteins. To understand the function of each, we constructed flies expressing Loqs-PA, Loqs-PB, or Loqs-PD. Loqs-PD promotes both endo- and exo-siRNA production by Dicer-2. Loqs-PA or Loqs-PB is required for viability, but the proteins are not fully redundant: a specific subset of miRNAs requires Loqs-PB. Surprisingly, Loqs-PB tunes where Dicer-1 cleaves pre-miR-307a, generating a longer miRNA isoform with a distinct seed sequence and target specificity. The longer form of miR-307a represses glycerol kinase and taranis mRNA expression. The mammalian Dicer-partner TRBP, a Loqs-PB homolog, similarly tunes where Dicer cleaves pre-miR-132. Thus, Dicer-binding partner proteins change the choice of cleavage site by Dicer, producing miRNAs with target specificities different from those made by Dicer alone or Dicer bound to alternative protein partners.Source
Cell. 2012 Oct 26;151(3):533-46. doi: 10.1016/j.cell.2012.09.027. Link to article on publisher's siteDOI
10.1016/j.cell.2012.09.027Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25939PubMed ID
23063653Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.cell.2012.09.027