Functional annotation of native enhancers with a Cas9-histone demethylase fusion
Authors
Kearns, Nicola A.Pham, Hannah
Tabak, Barbara
Genga, Ryan M. J.
Silverstein, Noah J.
Garber, Manuel
Maehr, Rene
UMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyProgram in Molecular Medicine
Diabetes Center of Excellence
Document Type
Journal ArticlePublication Date
2015-05-01Keywords
AnimalsBacterial Proteins
Caspase 9
Cells, Cultured
Embryonic Stem Cells
Enhancer Elements, Genetic
Gene Expression Regulation, Enzymologic
Genes, Reporter
Histone Demethylases
Histones
Mice
Neisseria meningitidis
Recombinant Proteins
Biochemistry, Biophysics, and Structural Biology
Bioinformatics
Computational Biology
Integrative Biology
Systems Biology
Metadata
Show full item recordAbstract
Understanding of mammalian enhancers is limited by the lack of a technology to rapidly and thoroughly test the cell type-specific function. Here, we use a nuclease-deficient Cas9 (dCas9)-histone demethylase fusion to functionally characterize previously described and new enhancer elements for their roles in the embryonic stem cell state. Further, we distinguish the mechanism of action of dCas9-LSD1 at enhancers from previous dCas9-effectors.Source
Nat Methods. 2015 May;12(5):401-3. doi: 10.1038/nmeth.3325. Epub 2015 Mar 16. Link to article on publisher's site
DOI
10.1038/nmeth.3325Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25938PubMed ID
25775043Related Resources
ae974a485f413a2113503eed53cd6c53
10.1038/nmeth.3325