Using Amplicon Deep Sequencing to Detect Genetic Signatures of Plasmodium vivax Relapse
Authors
Lin, Jessica T.Hathaway, Nicholas J
Saunders, David L.
Lon, Chanthap
Balasubramanian, Sujata
Kharabora, Oksana
Gosi, Panita
Sriwichai, Sabaithip
Kartchner, Laurel
Chuor, Char Meng
Satharath, Prom
Lanteri, Charlotte
Bailey, Jeffrey A.
Juliano, Jonathan J.
UMass Chan Affiliations
Program in Bioinformatics and Integrative BiologyDocument Type
Journal ArticlePublication Date
2015-09-15Keywords
CambodiaDNA, Protozoan
Gene Expression Regulation
Genetic Variation
Haplotypes
Humans
Malaria, Vivax
Merozoite Surface Protein 1
Microsatellite Repeats
Phylogeny
Plasmodium vivax
Recurrence
Plasmodium vivax
amplicon sequencing
deep sequencing
genetic diversity
hypnozoite
malaria
microsatellite
multiplicity of infection
pvmsp1
relapse
Bioinformatics
Computational Biology
Genetics
Genomics
Immunology of Infectious Disease
Parasitology
Population Biology
Metadata
Show full item recordAbstract
Plasmodium vivax infections often recur due to relapse of hypnozoites from the liver. In malaria-endemic areas, tools to distinguish relapse from reinfection are needed. We applied amplicon deep sequencing to P. vivax isolates from 78 Cambodian volunteers, nearly one-third of whom suffered recurrence at a median of 68 days. Deep sequencing at a highly variable region of the P. vivax merozoite surface protein 1 gene revealed impressive diversity-generating 67 unique haplotypes and detecting on average 3.6 cocirculating parasite clones within individuals, compared to 2.1 clones detected by a combination of 3 microsatellite markers. This diversity enabled a scheme to classify over half of recurrences as probable relapses based on the low probability of reinfection by multiple recurring variants. In areas of high P. vivax diversity, targeted deep sequencing can help detect genetic signatures of relapse, key to evaluating antivivax interventions and achieving a better understanding of relapse-reinfection epidemiology.Source
J Infect Dis. 2015 Sep 15;212(6):999-1008. doi: 10.1093/infdis/jiv142. Epub 2015 Mar 6. Link to article on publisher's siteDOI
10.1093/infdis/jiv142Permanent Link to this Item
http://hdl.handle.net/20.500.14038/25932PubMed ID
25748326Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1093/infdis/jiv142