Title

Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays

UMMS Affiliation

Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology

Publication Date

2010-06-09

Document Type

Article

Subjects

DNA, Circular; *Nanotechnology; Sequence Analysis, DNA

Disciplines

Bioinformatics | Computational Biology | Molecular Biology | Nanoscience and Nanotechnology

Abstract

We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.

DOI of Published Version

10.1021/nl1012147

Source

Nano Lett. 2010 Jun 9;10(6):2237-44. doi: 10.1021/nl1012147. Link to article on publisher's site

Journal/Book/Conference Title

Nano letters

Related Resources

Link to Article in PubMed

PubMed ID

20459065

Share

COinS