Title
Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays
UMMS Affiliation
Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology
Publication Date
2010-06-09
Document Type
Article
Subjects
DNA, Circular; *Nanotechnology; Sequence Analysis, DNA
Disciplines
Bioinformatics | Computational Biology | Molecular Biology | Nanoscience and Nanotechnology
Abstract
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
DOI of Published Version
10.1021/nl1012147
Source
Nano Lett. 2010 Jun 9;10(6):2237-44. doi: 10.1021/nl1012147. Link to article on publisher's site
Journal/Book/Conference Title
Nano letters
Related Resources
PubMed ID
20459065
Repository Citation
McNally, Ben; Singer, Alon; Yu, Zhiliang; Sun, Yingjie; Weng, Zhiping; and Meller, Amit, "Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays" (2010). Program in Bioinformatics and Integrative Biology Publications and Presentations. 36.
https://escholarship.umassmed.edu/bioinformatics_pubs/36