Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays
Department of Biochemistry and Molecular Pharmacology; Program in Bioinformatics and Integrative Biology
DNA, Circular; *Nanotechnology; Sequence Analysis, DNA
Bioinformatics | Computational Biology | Molecular Biology | Nanoscience and Nanotechnology
We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.
DOI of Published Version
Nano Lett. 2010 Jun 9;10(6):2237-44. doi: 10.1021/nl1012147. Link to article on publisher's site
McNally, Ben; Singer, Alon; Yu, Zhiliang; Sun, Yingjie; Weng, Zhiping; and Meller, Amit, "Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays" (2010). Program in Bioinformatics and Integrative Biology Publications and Presentations. 36.