UMMS Affiliation
Program in Bioinformatics and Integrative Biology; Program in Molecular Medicine; Garber Lab
Publication Date
2022-06-08
Document Type
Article
Disciplines
Bioinformatics | Congenital, Hereditary, and Neonatal Diseases and Abnormalities | Genetics and Genomics | Molecular Biology | Nervous System Diseases | Neuroscience and Neurobiology
Abstract
Despite advances in understanding the pathophysiology of Fragile X syndrome (FXS), its molecular basis is still poorly understood. Whole brain tissue expression profiles have proved surprisingly uninformative, therefore we applied single cell RNA sequencing to profile an FMRP deficient mouse model with higher resolution. We found that the absence of FMRP results in highly cell type specific gene expression changes that are strongest among specific neuronal types, where FMRP-bound mRNAs were prominently downregulated. Metabolic pathways including translation and respiration are significantly upregulated across most cell types with the notable exception of excitatory neurons. These effects point to a potential difference in the activity of mTOR pathways, and together with other dysregulated pathways, suggest an excitatory-inhibitory imbalance in the Fmr1-knock out cortex that is exacerbated by astrocytes. Our data demonstrate that FMRP loss affects abundance of key cellular communication genes that potentially affect neuronal synapses and provide a resource for interrogating the biological basis of this disorder.
Keywords
Neurons, Gene expression, Astrocytes, Messenger RNA, Mouse models, Protein translation, Mitochondria, Synapses
Rights and Permissions
Copyright: © 2022 Donnard et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI of Published Version
10.1371/journal.pgen.1010221
Source
Donnard E, Shu H, Garber M. Single cell transcriptomics reveals dysregulated cellular and molecular networks in a fragile X syndrome model. PLoS Genet. 2022 Jun 8;18(6):e1010221. doi: 10.1371/journal.pgen.1010221. PMID: 35675353; PMCID: PMC9212148. Link to article on publisher's site
Journal/Book/Conference Title
PLoS genetics
Related Resources
PubMed ID
35675353
Repository Citation
Donnard E, Shu H, Garber M. (2022). Single cell transcriptomics reveals dysregulated cellular and molecular networks in a fragile X syndrome model. Program in Bioinformatics and Integrative Biology Publications. https://doi.org/10.1371/journal.pgen.1010221. Retrieved from https://escholarship.umassmed.edu/bioinformatics_pubs/170
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Included in
Bioinformatics Commons, Congenital, Hereditary, and Neonatal Diseases and Abnormalities Commons, Genetics and Genomics Commons, Molecular Biology Commons, Nervous System Diseases Commons, Neuroscience and Neurobiology Commons
Comments
This article is based on a previously available preprint in bioRxiv.