Title

Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors

UMMS Affiliation

Program in Bioinformatics and Integrative Biology

Publication Date

2020-02-03

Document Type

Article

Disciplines

Biochemistry, Biophysics, and Structural Biology | Bioinformatics | Cell Biology | Computational Biology | Developmental Biology | Nucleic Acids, Nucleotides, and Nucleosides | Reproductive and Urinary Physiology

Abstract

PIWI-interacting RNAs (piRNAs) are a class of small non-coding RNAs essential for fertility. In adult mouse testes, most piRNAs are derived from long single-stranded RNAs lacking annotated open reading frames (ORFs). The mechanisms underlying how piRNA sequences are defined during the cleavages of piRNA precursors remain elusive. Here, we show that 80S ribosomes translate the 5'-proximal short ORFs (uORFs) of piRNA precursors. The MOV10L1/Armitage RNA helicase then facilitates the translocation of ribosomes into the uORF downstream regions (UDRs). The ribosome-bound UDRs are targeted by piRNA processing machinery, with the processed ribosome-protected regions becoming piRNAs. The dual modes of interaction between ribosomes and piRNA precursors underlie the distinct piRNA biogenesis requirements at uORFs and UDRs. Ribosomes also mediate piRNA processing in roosters and green lizards, implying that this mechanism is evolutionarily conserved in amniotes. Our results uncover a function for ribosomes on non-coding regions of RNAs and reveal the mechanisms underlying how piRNAs are defined.

Keywords

RNA, Spermatogenesis

DOI of Published Version

10.1038/s41556-019-0457-4

Source

Sun YH, Zhu J, Xie LH, Li Z, Meduri R, Zhu X, Song C, Chen C, Ricci EP, Weng Z, Li XZ. Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors. Nat Cell Biol. 2020 Feb;22(2):200-212. doi: 10.1038/s41556-019-0457-4. Epub 2020 Feb 3. Erratum in: Nat Cell Biol. 2020 Mar;22(3):353. PMID: 32015435. Link to article on publisher's site

Journal/Book/Conference Title

Nature cell biology

Related Resources

Link to Article in PubMed

PubMed ID

32015435

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